| Autor: |
Berezikov E; Hubrecht Laboratory, 3584 CT Utrecht, The Netherlands., van Tetering G, Verheul M, van de Belt J, van Laake L, Vos J, Verloop R, van de Wetering M, Guryev V, Takada S, van Zonneveld AJ, Mano H, Plasterk R, Cuppen E |
| Jazyk: |
angličtina |
| Zdroj: |
Genome research [Genome Res] 2006 Oct; Vol. 16 (10), pp. 1289-98. Date of Electronic Publication: 2006 Sep 05. |
| DOI: |
10.1101/gr.5159906 |
| Abstrakt: |
MicroRNAs are 20- to 23-nucleotide RNA molecules that can regulate gene expression. Currently > 400 microRNAs have been experimentally identified in mammalian genomes, whereas estimates go up to 1000 and beyond. Here we show that many more mammalian microRNAs exist. We discovered novel microRNA candidates using two approaches: testing of computationally predicted microRNAs by a modified microarray-based detection system, and cloning and sequencing of large numbers of small RNAs from different human and mouse tissues. Together these efforts experimentally identified 348 novel mouse and 81 novel human microRNA candidate genes. Most novel microRNAs candidates are not conserved beyond mammals, and ~10% are taxon-specific. Our analyses indicate that the entire microRNA repertoire is not remotely exhausted. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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