| Autor: |
Madabusi LV; Asuragen Discovery Services, Asuragen, Inc., Austin, TX, USA., Latham GJ, Andruss BF |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in enzymology [Methods Enzymol] 2006; Vol. 411, pp. 1-14. |
| DOI: |
10.1016/S0076-6879(06)11001-0 |
| Abstrakt: |
DNA microarrays enable insights into global gene expression by capturing a snapshot of cellular expression levels at the time of sample collection. Careful RNA handling and extraction are required to preserve this information properly, ensure sample-to-sample reproducibility, and limit unwanted technical variation in experimental data. This chapter discusses important considerations for "array-friendly" sample handling and processing from biosamples such as blood, formalin-fixed, paraffin-embedded samples, and fresh or flash-frozen tissues and cells. It also provides guidelines on RNA quality assessments, which can be used to validate sample preparation and maximize recovery of relevant biological information. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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