| Autor: |
Kal'nov SL; D I Ivanovsky Institute of Virology, Russian Academy of Medical Sciences. kalnov@narvac.com, Grigor'ev VB, Alekseev KP, Vlasova AN, Gibadulin RA, Pokidyshev AN, Balandina MV, Tsibezov VV, Verkhovskii OA |
| Jazyk: |
angličtina |
| Zdroj: |
Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2006 Jan; Vol. 141 (1), pp. 62-5. |
| DOI: |
10.1007/s10517-006-0094-3 |
| Abstrakt: |
Full-length Bos taurus PrPC protein was obtained in the eu- and prokaryotic expression systems. Immunoblotting and indirect enzyme immunoassay demonstrated high specificity and antigenic activity of full-length proteins in the reactions with monoclonal antibodies (anti-SAF-32 and VRQ-84). Membrane location of recombinant PrPC protein in insect cells was shown by immunofluorescent analysis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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