| Autor: |
Listerman I; Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany., Sapra AK, Neugebauer KM |
| Jazyk: |
angličtina |
| Zdroj: |
Nature structural & molecular biology [Nat Struct Mol Biol] 2006 Sep; Vol. 13 (9), pp. 815-22. Date of Electronic Publication: 2006 Aug 20. |
| DOI: |
10.1038/nsmb1135 |
| Abstrakt: |
Coupling between transcription and RNA processing is a key gene regulatory mechanism. Here we use chromatin immunoprecipitation to detect transcription-dependent accumulation of the precursor mRNA (pre-mRNA) splicing factors hnRNP A1, U2AF65 and U1 and U5 snRNPs on the intron-containing human FOS gene. These factors were poorly detected on intronless heat-shock and histone genes, a result that opposes direct recruitment by RNA polymerase II (Pol II) or the cap-binding complex in vivo. However, an observed RNA-dependent interaction between U2AF65 and active forms of Pol II may stabilize U2AF65 binding to intron-containing nascent RNA. We establish chromatin-RNA immunoprecipitation and show that FOS pre-mRNA is cotranscriptionally spliced. Notably, the topoisomerase I inhibitor camptothecin, which stalls elongating Pol II, increased cotranscriptional splicing factor accumulation and splicing in parallel. This provides direct evidence for a kinetic link between transcription, splicing factor recruitment and splicing catalysis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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