| Autor: |
Friend LD; Program in Immunology, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA., Shah DD, Deppong C, Lin J, Bricker TL, Juehne TI, Rose CM, Green JM |
| Jazyk: |
angličtina |
| Zdroj: |
The Journal of experimental medicine [J Exp Med] 2006 Sep 04; Vol. 203 (9), pp. 2121-33. Date of Electronic Publication: 2006 Aug 14. |
| DOI: |
10.1084/jem.20052230 |
| Abstrakt: |
Activation of naive T cells requires the integration of signals through the antigen receptor and CD28. Although there is agreement on the importance of CD28, there remains controversy on the mechanism by which CD28 regulates T cell function. We have generated a gene-targeted knockin mouse expressing a mutation in the C-terminal proline-rich region of the cytoplasmic tail of CD28. Our analysis conclusively showed that this motif is essential for CD28-dependent regulation of interleukin 2 secretion and proliferation. In vivo analysis revealed that mutation of this motif-dissociated CD28-dependent regulation of cellular and humoral responses in an allergic airway inflammation model. Furthermore, we find an important gene dosage effect on the phenotype of the mutation and provide a mechanistic explanation for the conflicting data on the significance of this motif in CD28 function. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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