Analysis of variation in batches of armadillo-derived Mycobacterium leprae by immunoblotting.

Autor: Ibrahim MA; Laboratory for Leprosy and Mycobacterial Research, National Institute for Medical Research, Mill Hill, London, U.K., Lamb FI, Colston MJ
Jazyk: angličtina
Zdroj: International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association [Int J Lepr Other Mycobact Dis] 1990 Mar; Vol. 58 (1), pp. 73-7.
Abstrakt: Several batches of cell-free extracts of armadillo-derived Mycobacterium leprae were analyzed by SDS-PAGE and by immunoblotting with monoclonal antibodies. The presence or absence of protease inhibitors had a profound effect on the protein antigens, particularly the 65-kDa antigen. In the absence of protease inhibitors, there were both quantitative and qualitative differences between the different batches of M. leprae extracts.
Databáze: MEDLINE