| Autor: |
Rodziewicz L; Pracownia Badań Chemicznych Srodków Spozywczych, Zakład Higieny Weterynaryjnej, Białystok. rodziewicz@wiw.bianet.com.pl, Zawadzka I |
| Jazyk: |
polština |
| Zdroj: |
Roczniki Panstwowego Zakladu Higieny [Rocz Panstw Zakl Hig] 2006; Vol. 57 (1), pp. 31-7. |
| Abstrakt: |
A liquid chromatographic method with mass spectrometric determination and identification (LC-MS/MS) for the determination of chloramphenicol (CAP) in tissues of animal origin was presented. Homogenized samples were extracted with ethyl acetate and evaporated to dryness followed by a clean-up using the liquid-liquid distribution between acetonitryle and hexane. Chloramphenicol was determined by LC-MS-ESI-MS in negative mode. There was used Phenomenex column with the mixture of acetonitrile-water as a mobile phase. The method was validated according to the criteria of Decision Commission No 2002/657/EC. Samples were fortified at CAP levels between 0.1 and 0.45 ng/g with 5d-CAP as internal standard. Recoveries for the level 0.3 ng/g were in the range 76.3-100.3%. Limit of decision (CCalpha) was between 0.137-0.205 ng/g. Limit of quantification (LOQ)--0.1 ng/g. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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