| Autor: |
Steighner RJ; Department of Pharmacology and Toxicology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298., Povirk LF |
| Jazyk: |
angličtina |
| Zdroj: |
Mutation research [Mutat Res] 1990 Feb; Vol. 240 (2), pp. 93-100. |
| DOI: |
10.1016/0165-1218(90)90012-q |
| Abstrakt: |
Previous studies have revealed bleomycin to be a potent base-substitution mutagen in repackaged phage lambda. In order to assess the role of apurinic/apyrimidinic (AP) sites in bleomycin-induced mutagenesis, bleomycin-damaged lambda DNA was treated with putrescine or endonuclease IV to effect cleavage of bleomycin-induced AP sites. The DNA was then packaged, the phage grown in SOS-induced E. coli, and the frequency of clear-plaque mutants in the progeny was determined. Bleomycin-induced mutagenesis was decreased approx. 2-fold by treating the DNA with putrescine, but was unaffected by endonuclease IV. The results are consistent with the production of bleomycin-induced mutation at certain AP sites having a closely opposed single-strand break, since such sites are cleaved by putrescine but not by endonuclease IV. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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