Establishment and characterization of amylase-producing lung adenocarcinoma cell line, IMEC-2.

Autor:	Yokouchi H; First Department of Medicine, Hokkaido University School of Medicine, North 15, West 7, Kita-ku, Sapporo, Japan. yokouchi@med.hokudai.ac.jp, Yamazaki K, Asahina H, Shigemura M, Moriyama T, Takaoka K, Moriya J, Itoh T, Kinoshita I, Dosaka-Akita H, Tsutsumi Y, Nishimura M
Jazyk:	angličtina
Zdroj:	Anticancer research [Anticancer Res] 2006 Jul-Aug; Vol. 26 (4B), pp. 2821-7.
Abstrakt:	Background: Few studies have successfully established an amylase-producing lung cancer cell line or have examined its cytological, biochemical and biological features. Patients and Methods: Cancer cells, isolated from pleural effusion using a gradient method, were cultivated. Results: Amylase production from the newly established cell line was confirmed by positive staining for alpha-amylase and increased amylase levels in the culture supernatant. Electron microscopy revealed zymogen granule-like structures. Sialylation of salivary-type amylase was confirmed directly from the cell line by examining the neuraminidase sensitivity and amylase elution profile under high-performance liquid chromatography. Neither EGFR or KRAS mutation were found. Conclusion: This cell line offers a useful tool for analyzing the pathogenesis and pathophysiology of amylase-producing lung cancers. Moreover, it might be useful for probing the metastasis and invasiveness of lung cancer cells and for developing an early diagnostic method based on sialylated salivary-amylase production.
Databáze:	MEDLINE
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