| Autor: |
Alves C; Universidade de São Paulo, Instituto de Química de São Carlos Av. Trabalhador Sãocarlense 400, Caixa Postal 780, CEP 13566-590, São Carlos (SP), Brazil., Fernandes C, Dos Santos Neto AJ, Rodrigues JC, Costa Queiroz ME, Lanças FM |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatographic science [J Chromatogr Sci] 2006 Jul; Vol. 44 (6), pp. 340-6. |
| DOI: |
10.1093/chromsci/44.6.340 |
| Abstrakt: |
Solid-phase microextraction (SPME)-liquid chromatography (LC) is used to analyze tricyclic antidepressant drugs desipramine, imipramine, nortriptyline, amitriptyline, and clomipramine (internal standard) in plasma samples. Extraction conditions are optimized using a 2(3) factorial design plus a central point to evaluate the influence of the time, temperature, and matrix pH. A Polydimethylsiloxane-divinylbenzene (60-mum film thickness) fiber is selected after the assessment of different types of coating. The chromatographic separation is realized using a C(18) column (150 x 4.6 mm, 5-microm particles), ammonium acetate buffer (0.05 mol/L, pH 5.50)-acetonitrile (55:45 v/v) with 0.1% of triethylamine as mobile phase and UV-vis detection at 214 nm. Among the factorial design conditions evaluated, the best results are obtained at a pH 11.0, temperature of 30 degrees C, and extraction time of 45 min. The proposed method, using a lab-made SPME-LC interface, allowed the determination of tricyclic antidepressants in in plasma at therapeutic concentration levels. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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