| Autor: |
Farid YY; Department of Pathological Biochemistry, Royal Infirmary, Glasgow G4 OSF, Scotland, UK., Watson ID, Stewart MJ |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 1983; Vol. 1 (1), pp. 55-63. |
| DOI: |
10.1016/0731-7085(83)80008-9 |
| Abstrakt: |
A high-performance liquid chromatographic assay for methotrexate and its metabolites, 7-hydroxy-methotrexate, 4-amino-4-deoxy-N10-methyl-pteroic acid and 7-hydroxy-4-amino-4-deoxy-N10-methyl-pteroic acid in the range 10 microg/l to 50 mg/l (2.2 x 10(-8) to 1.1. x 10(-4)M) has been developed using L-tryptophyl-L-glutamic acid as internal standard. Extraction was performed using an anion exchange resin (Dowex 1-X2) with subsequent ion-pair chromatography of the appropriate eluent fraction. The method has been found to be sensitive and precise for the analysis of both serum and urine, and may also be used for the quantitation of polyglutamyl metabolites. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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