| Autor: |
Yang ZY; Division of Applied Life Science, Graduate School of Gyeongsan National University, Chinju, Gyeongnam 660-701, South Korea., Kolosova AY, Shim WB, Chung DH |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of agricultural and food chemistry [J Agric Food Chem] 2006 Jun 28; Vol. 54 (13), pp. 4551-6. |
| DOI: |
10.1021/jf0606196 |
| Abstrakt: |
To detect the organophosphorus (OP) pesticide pirimiphos-methyl in grain samples, a monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) was developed and optimized. By the active esters method, pirimiphos-methyl hapten A was conjugated to keyhole limpet hemocyanin to be used as the immunogen for the production of monoclonal antibodies, and pirimiphos-methyl hapten B was conjugated to ovalbumin to be used as coating antigen. By using the monoclonal antibody and the coating antigen, an IC-ELISA has been developed. Under the established optimized conditions, the IC-ELISA showed an IC50 of 4.2 ng/mL with a detection limit of 0.07 ng/mL. The IC-ELISA showed negligible cross-reactivity with other OP pesticides except with pirimiphos-ethyl. Recoveries of pirimiphos-methyl from spiked grain samples ranged from 83 to 96%. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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