Microtubule-associated protein-4 (MAP-4) inhibits microtubule-dependent distribution of mRNA in isolated neonatal cardiocytes.
| Autor: | Scholz D; Gazes Cardiac Research Institute, Cardiology Division, Medical University of South Carolina, and Department of Veterans Affairs Medical Center, Charleston, SC 29401, United States. scholzd@musc.edu, McDermott P, Garnovskaya M, Gallien TN, Huettelmaier S, DeRienzo C, Cooper G 4th |
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| Jazyk: | angličtina |
| Zdroj: | Cardiovascular research [Cardiovasc Res] 2006 Aug 01; Vol. 71 (3), pp. 506-16. Date of Electronic Publication: 2006 Jun 05. |
| DOI: | 10.1016/j.cardiores.2006.04.001 |
| Abstrakt: | Objectives: Active mRNA distribution in the form of ribonucleoprotein particles moving along microtubules has been shown in several cell types, but not yet in cardiocytes. This study addresses two hypotheses: 1) a similar mRNA distribution mechanism operates in cardiocytes; 2) decoration of microtubules with microtubule-associated proteins compromises this distribution. Methods: To visualize ribonucleoproteins in cultured neonatal rat cardiocytes, they were transfected with vectors encoding zipcode binding protein-1 and Staufen fused with GFP. The velocity of microtubular transport and elongation were calculated on time-lapse confocal pictures. Results: ZBP-1 and Staufen labeled particles co-localized with each other and with microtubules and moved along microtubules over a distance of 1-20 microm with a mean speed of 80 nm/s. The average speed decreased about 50% after decoration of microtubules by adenoviral microtubule-associated protein-4 (MAP-4). The elongation speed measured using the GFP-tagged end-binding protein-1 exceeded 200 nm/s and was not influenced by MAP-4. Conclusions: We demonstrate for the first time ribonucleoprotein particles in cardiocytes, their microtubular-related movement, and its inhibition (but not of the microtubular elongation), by the MAP-4 decoration of microtubules. |
| Databáze: | MEDLINE |
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