| Autor: |
Southern MM; Department of Biological Sciences, University of Warwick, UK., Brown PE, Hall A |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2006; Vol. 323, pp. 293-305. |
| DOI: |
10.1385/1-59745-003-0:293 |
| Abstrakt: |
Luciferase is the ideal reporter gene to provide temporal and spatial information on promoter activity in Arabidopsis and other eukaryotes; the noninvasive detection of luminescence and short half-life of luciferase activity allow repeated measurements of individual seedlings over several days to assay dynamic changes in gene expression. Transgenic or transiently transformed plants with a luciferase gene under the control of a promoter of interest are required. Detection of the low level of luminescence produced by the luciferase gene in Arabidopsis requires the use of low light detecting charge-coupled device (CCD) cameras or scintillation counters. This chapter contains protocols on assaying and imaging luciferase in vivo and the automation for high-resolution timecourses. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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