Autor: |
Pascon RC; Alellyx Applied Genomics, Techno Park (Rod. Anhanguera Km 104), Rua James Clerk Maxwell 320, 13069-380, Campinas, SP, Brazil., Kitajima JP, Breton MC, Assumpção L, Greggio C, Zanca AS, Okura VK, Alegria MC, Camargo ME, Silva GG, Cardozo JC, Vallim MA, Franco SF, Silva VH, Jordão H Jr, Oliveira F, Giachetto PF, Ferrari F, Aguilar-Vildoso CI, Franchiscini FJ, Silva JM, Arruda P, Ferro JA, Reinach F, da Silva AC |
Jazyk: |
angličtina |
Zdroj: |
Virus genes [Virus Genes] 2006 Jun; Vol. 32 (3), pp. 289-98. |
DOI: |
10.1007/s11262-005-6913-1 |
Abstrakt: |
The Citrus leprosis disease (CiL) is associated to a virus (CiLV) transmitted by Brevipalpus spp. mites (Acari: Tenuipalpidae). CiL is endemic in Brazil and its recently spreading to Central America represents a threat to citrus industry in the USA. Electron microscopy images show two forms of CiLV: a rare nuclear form, characterized by rod-shaped naked particle (CiLV-N) and a common cytoplasmic form (CiLV-C) associated with bacilliform-enveloped particle and cytoplasmic viroplasm. Due to this morphological feature, CiLV-C has been treated as Rhabdovirus-like. In this paper we present the complete nucleotide sequence and genomic organization of CiLV-C. It is a bipartite virus with sequence similarity to ssRNA positive plant virus. RNA1 encodes a putative replicase polyprotein and an ORF with no known function. RNA2 encodes 4 ORFs. pl5, p24 and p61 have no significant similarity to any known proteins and p32 encodes a protein with similarity to a viral movement protein. The CiLV-C sequences are associated with typical symptoms of CiL by RT-PCR. Phylogenetic analysis suggests that CiLV-C is probably a member of a new family of plant virus evolutionarily related to Tobamovirus. |
Databáze: |
MEDLINE |
Externí odkaz: |
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