| Autor: |
Bielanski A; Agriculture Canada, Animal Diseases Research Institute, NEPEAN, P.O.Box 11300, Station H, Nepean, Ontario, Canada K2H 8P9., Eastman P, Hare WC |
| Jazyk: |
angličtina |
| Zdroj: |
Theriogenology [Theriogenology] 1991 Jul; Vol. 36 (1), pp. 33-40. |
| DOI: |
10.1016/0093-691x(91)90431-c |
| Abstrakt: |
The fertilizing capacity of transitorily acidified semen was investigated with respect to using acidification as a method for destroying or inactivating acid labile pathogenic microorganisms in semen prior to freezing. Ejaculates diluted 1:1 with phosphate buffered saline (PBS) were acidified to pH 5.0 for 2 or 5 minutes before being returned to their original pH. They were then frozen by commercial methods and used to artificially inseminate superovulated heifers. A total 739 ova and embryos was collected from 119 inseminated donors. The fertilization rate was 88%, and 78% of the embryos were of transferable quality. Semen acidification had no apparent effect on the post-thaw percentage of intact acrosomes. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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