| Autor: |
Jacek Z; Akademia Medyczna w Poznaniu, Pracownia Dializ, Klinika Kardiologii i Nefrologii Dzieciecej. J.Zachwieja@mp.pl, Marcin Z, Maria LS, Ewa S, Alfred W, Aldona S |
| Jazyk: |
polština |
| Zdroj: |
Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego [Pol Merkur Lekarski] 2006 Feb; Vol. 20 (116), pp. 203-6. |
| Abstrakt: |
Recently it was shown that oxidative stress has a negative influence on immunological response. The aim of the study was to investigate the influence of N-acetylcysteine on intracellular oxidative stress and cytokine synthesis in peripheral blood lymphocytes in children with end stage renal disease (ESRD). MATERIAL AND METHODS. We examined 25 children (age 2-16 ys.) with ESRD. To determine oxidative stress we used dihydrorhodamine 123 (DHR). Intracellular oxidation of DHR in T. lymphocytes reflected intracellular oxidative stress within cells. The intracellular synthesis of cytokines (IL-2, IFN-r, IL-4, IL-6) was detected with flow cytometty RESULTS. We found lower intracellular stress and higher cytokines synthesis after incubation with N-acetylcysteine (NA C) in all subpopulations of lymphocytes. CONCLUSIONS. Our results shows that patients with ESRD present increased oxidative stress in T lymphocytes which may lead to decreased cytokines synthesis and abnormal immune response. NAC has favorable influence on these abnormalities. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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