| Autor: |
Gong H; BioMEMS Laboratory, School of Mechanical and Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798. mhqgong@ntu.edu.sg, Ramalingam N, Chen L, Che J, Wang Q, Wang Y, Yang X, Yap PH, Neo CH |
| Jazyk: |
angličtina |
| Zdroj: |
Biomedical microdevices [Biomed Microdevices] 2006 Jun; Vol. 8 (2), pp. 167-76. |
| DOI: |
10.1007/s10544-006-7712-8 |
| Abstrakt: |
A microfluidic biochip for conducting an array of polymerase chain reaction (PCR) simultaneously was fabricated to understand the microfluidic loading process of PCR solution into microfabricated glass reaction chambers. The geometrical factors of the microfluidic structure, including the shape and depth of the microchamber, shape and size of the microchannels were investigated on the formation of air bubbles trapped within the microchamber during the PCR solution loading process. Furthermore, the effects of surface properties of the microfluidic structure, including hydrophilicity of the microchamber and inlet channel, and hydrophobicity of the outlet channel, on the loading of PCR solution, especially on the formation of air bubbles were studied. As a result, the surface wetting property of the microchamber was found to be the main reason for the formation of the air bubbles inside the microchamber during the loading of PCR solution in the biochips. A solution to avoid the air trapping has been proposed and investigated. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Full text from SpringerLink