| Abstrakt: |
The possibility of elimination of C2-metabolism limitation under Acinetobacter sp. B-7005 (producer of exopolysaccharide ethapolan) cultivation on ethanol-glucose mixture was shown. Cultivation of these bacteria on the nonbuffered medium was accompanied by pH decrease (5.6-4.7), acetate accumulation (25-45 mM), drop in the biomass and exopolysaccharide concentration. The decrease of the ammonium nitrate content (to 0.3 g/l) in the medium, its replacement by nitrate, the increase of the pantotenate calcium concentration to 0.0009%, potassium and magnesium cations to 100 and 5-10 mM, application of the inoculum grown on acetate or ethanol, the decrease of the substrates initial concentration to 0.25% permitted a prevention of acetate accumulation and realization of ethanol synthesis on ethanol-glucose mixture on the inbuffered medium. |
