| Autor: |
Berg JP; Hormone Laboratory, Aker Hospital, Oslo, Norway., Sørnes G, Torjesen PA, Haug E |
| Jazyk: |
angličtina |
| Zdroj: |
Molecular and cellular endocrinology [Mol Cell Endocrinol] 1991 Apr; Vol. 76 (1-3), pp. 201-6. |
| DOI: |
10.1016/0303-7207(91)90274-v |
| Abstrakt: |
A rat thyroid cell line (FRTL-5) was used to study the effect of cholecalciferols on cAMP production. The active cholecalciferol metabolite, calcitriol, caused a reduction in basal and thyrotropin (TSH)-stimulated cAMP production. The inhibitory effects were demonstrated after 1 and 2 days, respectively. The maximum effect on both basal and TSH-stimulated cAMP production was observed after 3-4 days of treatment. The effect was detectable at 10(-10) and maximal at 10(-8) mol/l. Calcitriol was about 300 times more potent than calcidiol in attenuating cAMP production, whereas (24R)-hydroxycalcidiol in concentrations up to 3 x 10(-8) mol/l had no effect. After removal of added calcitriol the cAMP response to TSH returned to normal within 8 days. Calcitriol (10(-8) mol/l) also inhibited cell growth. Our results show that calcitriol at physiological concentrations inhibits both basal and TSH-stimulated cAMP production in rat thyroid cells. This indicates that calcitriol may modulate the effect of TSH on thyroid function and growth. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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