| Autor: |
Loewus FA; Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164., Baldi BG, Franceschi VR, Meinert LD, McCollum JJ |
| Jazyk: |
angličtina |
| Zdroj: |
Plant physiology [Plant Physiol] 1985 Jul; Vol. 78 (3), pp. 652-4. |
| DOI: |
10.1104/pp.78.3.652 |
| Abstrakt: |
4-Methylmorpholine N-oxide monohydrate (MMNO.H(2)O), a potent solvent for polysaccharides, is an effective vehicle for release of membrane-enclosed male gametophytes (sporoplasts) from spore walls. This release occurs in minutes when pollen (Lilium longiflorum Thunb.) is suspended in a melt of MMNO.H(2)O at 75 degrees C. Continued heating at 75 degrees C leads to distintegration of the exine ;shell' which coalesces into immiscible globules in the MMNO melt. These observations provide a general procedure for preparation of pollen sporoplasts and sporoplast outer membranes, and offer a new method for dissolving the sporopollenin component of the spore wall. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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