Autor: |
Ikuma H; Department of Cellular and Molecular Biology, Division of Biological Sciences, University of Michigan, Ann Arbor, Michigan 48109., Tetley RM |
Jazyk: |
angličtina |
Zdroj: |
Plant physiology [Plant Physiol] 1976 Sep; Vol. 58 (3), pp. 320-3. |
DOI: |
10.1104/pp.58.3.320 |
Abstrakt: |
Acid extracts from tissues of two solanaceous plants were found to contain a heat-labile, nondialyzable factor which hydrolyzes nucleoside di- and triphosphates to nucleoside monophosphates. This acid-resistant factor shows optimal ATP-hydrolyzing activity at pH 5, whereas practically no activity was detected below pH 3 and above pH 9. It does not hydrolyze sugar phosphates, nucleoside monophosphates, uridine diphosphoglucose, and phosphoenolpyruvate. In order to estimate quantitatively the amount of nucleoside di- and triphosphates in a plant extract, care must be taken to circumvent possible interference by this factor. This is achieved by carefully maintaining the extract below pH 3. |
Databáze: |
MEDLINE |
Externí odkaz: |
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