Methanogenesis is Ca2+ dependent in Methanothermobacter thermautotrophicus strain DeltaH.

Autor: Vancek M; Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovak Republic., Vidová M, Majerník AI, Smigán P
Jazyk: angličtina
Zdroj: FEMS microbiology letters [FEMS Microbiol Lett] 2006 May; Vol. 258 (2), pp. 269-73.
DOI: 10.1111/j.1574-6968.2006.00232.x
Abstrakt: The effect of Ca2+ ions on methanogenesis and growth of Methanothermobacter thermautotrophicus was investigated. The calcium chelator ethylene glycol bis(2-aminoethylether)-N,N,N',N'-tetra-acetic acid, calcium ionophore A23187 and ruthenium red all inhibited growth of this strain. Methane formation was strongly dependent on the external Ca2+ concentration in a resting cell suspension. In addition, methanogenesis of Ca2+ preloaded cells was stimulated by 400%. Inhibitor studies revealed that Co2+ and Ni2+, inorganic antagonists of Ca2+ transport, strongly inhibited methanogenesis in these cells. Interestingly, our findings imply that one of the enzymes of methanogenesis might catalyse a Ca2+ -dependent step and allow a direct activation of methanogenesis by Ca2+ ions.
Databáze: MEDLINE