| Autor: |
César Ida C; Department of Pharmaceutical Products, Faculty of Pharmacy, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil. isaccesar@bol.com.br, Braga FC, Soares CD, Nunan Ede A, Pianetti GA, Condessa FA, Barbosa TA, Campos LM |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2006 May 19; Vol. 836 (1-2), pp. 74-8. Date of Electronic Publication: 2006 Apr 03. |
| DOI: |
10.1016/j.jchromb.2006.03.030 |
| Abstrakt: |
Isoflavones are widely used as an alternative treatment to hormone replacement therapy and also for prevention of several chronic diseases, including cancers. Genistein, daidzein and glycitein are the most abundant isoflavone aglycones found in soy extracts, where they also occur as glycosides. This paper describes the development and validation of an isocratic reversed-phase HPLC (RP-HPLC) method for the analysis of isoflavone aglycones, released after acid hydrolysis of soy dry extracts, used as pharmaceutical raw material. The quantification was carried out in a C(18) endcapped column, using a mobile phase composed of 0.1% acetic acid and methanol (52:48), at a flow rate of 1.0 ml/min and diode array detection (DAD) at 254 nm. The method showed to be linear (r(2)>0.99), precise (R.S.D.<2%), accurate (recovery of 98.88% for daidzein and 98.12% for genistein), robust and specific. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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