| Autor: |
Bogle RG; Section of Vascular Biology, MRC Clinical Research Centre, Harrow, U.K., Coade SB, Moncada S, Pearson JD, Mann GE |
| Jazyk: |
angličtina |
| Zdroj: |
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1991 Oct 31; Vol. 180 (2), pp. 926-32. |
| DOI: |
10.1016/s0006-291x(05)81154-4 |
| Abstrakt: |
The effects of bradykinin and ATP on L-arginine transport and nitric oxide (NO) production were studied in porcine aortic endothelial cells cultured and perfused on microcarriers and deprived of L-arginine for 24 h. Stimulation of cells with bradykinin (100 nM) or ATP (100 microM) resulted in a rapid increase in L-arginine uptake and NO release. In the presence of nitro-L-arginine (100 microM), an inhibitor of NO synthase, the stimulatory effect of bradykinin on L-arginine uptake was partially inhibited while NO release was completely abolished. Nitro-L-arginine alone was not an inhibitor of basal L-arginine transport, suggesting that its inhibitory action was not directly on the L-arginine transporter but a result of the inhibition of NO generation. These data indicate that during agonist-stimulated NO production there is a concomitant increase in the transport of L-arginine into endothelial cells providing a mechanism for the continual generation of NO. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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