| Autor: |
Allison RF; Department of Plant Pathology, North Carolina State University, Raleigh, NC 27695., Sorenson JC, Kelly ME, Armstrong FB, Dougherty WG |
| Jazyk: |
angličtina |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1985 Jun; Vol. 82 (12), pp. 3969-72. |
| DOI: |
10.1073/pnas.82.12.3969 |
| Abstrakt: |
The nucleotide sequence of the 3'-terminal portion of the tobacco etch virus (TEV) genome was determined. The 2324-nucleotide sequence represented approximately one-fourth of the TEV genome and included the capsid protein gene and flanking regions. An open reading frame of 2135 nucleotides and an untranslated region of 189 nucleotides adjacent to a polyadenylate tract were identified. The sequence began within an open reading frame, indicating that the initiation codon was upstream of the available sequence data. The sequence of the 20 NH(2)-terminal amino acids of the TEV capsid protein was established chemically. An identical amino acid sequence, predicted from the nucleotide sequence, was located, commencing at amino acid - 263. These data indicated that maturation of the capsid protein required a post-translational cleavage of a larger protein precursor, with a probable cleavage site between the amino acids glutamine and glycine. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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