A unique molten globule state occurs during unfolding of cytochrome c by LiClO4 near physiological pH and temperature: structural and thermodynamic characterization.

MG state, is the same as that observed for each transition between native and MG states induced by other solvent conditions. -->
Substance Nomenclature: 0 (Lithium Compounds)
0 (Perchlorates)
9007-43-6 (Cytochromes c)
Q86SE98C9C (lithium perchlorate)
Entry Date(s): Date Created: 20060406 Date Completed: 20060530 Latest Revision: 20171116
Update Code: 20231215
DOI: 10.1021/bi052357r
PMID: 16584204
Autor: Moza B; Department of Biosciences, Jamia Millia Islamia, Jamia Nagar, New Delhi, 110 025, India., Qureshi SH, Islam A, Singh R, Anjum F, Moosavi-Movahedi AA, Ahmad F
Jazyk: angličtina
Zdroj: Biochemistry [Biochemistry] 2006 Apr 11; Vol. 45 (14), pp. 4695-702.
DOI: 10.1021/bi052357r
Abstrakt: We have carried out denaturation studies of bovine cytochrome c (cyt c) by LiClO4 at pH 6.0 and 25 degrees C by observing changes in difference molar absorbance at 400 nm (Deltaepsilon400), mean residue ellipticities at 222 nm ([theta]222) and difference mean residue ellipticity at 409 nm (Delta[theta]409). The denaturation is a three-step process when measured by Deltaepsilon400 and Delta[theta]409, and it is a two-step process when monitored by [theta]222. The stable folding intermediate state has been characterized by near- and far-UV circular dichroism, tryptophan fluorescence, 8-anilino-1-naphthalene sulfonic acid (ANS) binding, and intrinsic viscosity measurements. A comparison of the conformational and thermodynamic properties of the LiClO4-induced molten globule (MG) state with those induced by other solvent conditions (e.g., low pH, LiCl, and CaCl2) suggests that LiClO4 induces a unique MG state, i.e., (i) the core in the LiClO4-induced state retains less secondary and tertiary structure than that in the MG states obtained in other solvent conditions, and (ii) the thermodynamic stability associated with the LiClO4-induced process, native state <--> MG state, is the same as that observed for each transition between native and MG states induced by other solvent conditions.
Databáze: MEDLINE
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