| Autor: |
van Loon AE; Ecole Normale Superieure, Laboratoire de Biologie Cellulaire et Moleculaire, Lyon, France., Colas P, Goedemans HJ, Néant I, Dalbon P, Guerrier P |
| Jazyk: |
angličtina |
| Zdroj: |
The EMBO journal [EMBO J] 1991 Nov; Vol. 10 (11), pp. 3343-9. |
| DOI: |
10.1002/j.1460-2075.1991.tb04898.x |
| Abstrakt: |
We have cloned and sequenced the cDNAs encoding Patella vulgata cyclins A and B. The cDNA clones contain an open reading frame of 426 and 408 amino acids respectively, which present similarity with cyclins from other species. Cyclin A and B RNAs are present as polyadenylated and non-polyadenylated RNA in prophase oocytes and are completely polyadenylated in metaphase I. During the first cleavages after fertilization the level of cyclin A and B mRNAs is high and drops when the free swimming stage is reached. Using p13suc1-Sepharose bead precipitation we demonstrate that cyclin synthesis is triggered during maturation and that inhibition of protein synthesis makes the cyclins disappear rapidly from the metaphase I oocytes, which shift to interphase condition. By microinjecting antisense oligonucleotides into metaphase I oocytes, we demonstrate that in vivo ablation of cyclin A and B messengers together gives the same result, whereas microinjection of only one oligonucleotide does not show any effect. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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