Confinement and clearance of OCT4 in the porcine embryo at stereomicroscopically defined stages around gastrulation.

Autor: Vejlsted M; Department of Animal and Veterinary Basic Sciences, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark. mov@kvl.dk, Offenberg H, Thorup F, Maddox-Hyttel P
Jazyk: angličtina
Zdroj: Molecular reproduction and development [Mol Reprod Dev] 2006 Jun; Vol. 73 (6), pp. 709-18.
DOI: 10.1002/mrd.20461
Abstrakt: In the areas of developmental biology and embryonic stem cell research, reliable molecular markers of pluripotency and early lineage commitment are sparse in large animal species. In this study, we present morphological and immunohistochemical findings on the porcine embryo in the period around gastrulation, days 8-17 postinsemination, introducing a stereomicroscopical staging system in this species. In embryos at the expanding hatched blastocyst stage, OCT4 is confined to the inner cell mass. Following detachment of the hypoblast, and formation of the embryonic disk, this marker of pluripotency was selectively observed in the epiblast. A prominent crescent-shaped thickening at the posterior region of the embryonic disk marked the first polarization within this structure reflecting incipient cell ingression. Following differentiation of the epiblast, clearance of OCT4 from the three germ layers was observed at defined stages, suggesting correlations to lineage specification. In the endoderm, clearance of OCT4 was apparent from early during its formation at the primitive streak stage. The endoderm harbored progenitors of the "fourth germ layer," the primordial germ cells (PGCs), the only cells maintaining expression of OCT4 at the end of gastrulation. In the ectodermal and mesodermal cell lineages, OCT4 became undetectable at the neural groove and somite stage, respectively. As in the mouse, PGCs showed onset of c-kit expression when located in extraembryonal compartments. They appeared to follow the endoderm during extraembryonal allocation and the mesoderm on return to the genital ridge.
(Copyright 2006 Wiley-Liss, Inc.)
Databáze: MEDLINE
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