Prospective flow cytometric evaluation of nucleated red blood cells in cord blood units and relationship with nucleated and CD34(+) cell quantification.

Autor: Larghero J; Cell Therapy Unit and Bone Marrow Transplant Department, Saint-Louis Hospital, Paris, France., Rea D, Brossard Y, Van Nifterik J, Delasse V, Robert I, Biscay N, Chantre E, Raffoux E, Socié G, Gluckman E, Benbunan M, Marolleau JP
Jazyk: angličtina
Zdroj: Transfusion [Transfusion] 2006 Mar; Vol. 46 (3), pp. 403-6.
DOI: 10.1111/j.1537-2995.2006.00736.x
Abstrakt: Background: Cord blood (CB) represents an alternate source of stem cells in transplantation. Nucleated red blood cells (NRBCs) are a physiological subset of CB population. Although it is important to have an accurate estimate of CD34(+) cell number, NRBCs could compromise white blood cell count and interfere with CD34(+) cell quantification.
Study Design and Methods: A total of 826 CB units were analyzed for total nucleated cells (TNCs), NRBCs, and CD34(+) cells by flow cytometry. NRBCs were also counted conventionally by manual microscopy. Percentages of CD34(+) cells corrected by NRBC count (CD34+c) were determined as follows: %CD34+c = CD34(+)/CD45(+) (x10(6))/(TNCs (x10(8)) - NRBCs (x10(8))).
Results: The mean percentages of CD34+ cells and NRBCs were 0.27 percent (range, 0.01%-1.25%) and 7.64 percent (range, 0.13%-84%), respectively. Comparison between flow cytometric and microscopic NRBC count showed a regression of y = 0.685 + 0.719x and a coefficient of determination of r(2) = 0.721. When corrected with NRBC count, the mean percentage of CD34(+) c cells was 0.295 percent (p = 0.0008 compared with CD34(+)%) and mean TNCc count was 14.8 x 10(8) (p < 10(-4) compared to TNC count).
Conclusion: The determination of NRBCs with a flow cytometric method might represent a new strategy for providing satisfactory quality assurance controls of CB products.
Databáze: MEDLINE
Externí odkaz: