| Autor: |
Constanzer ML; Merck Sharp & Dohme Research Laboratories, West Point, PA 19486., Matuszewski BK, Bayne WF |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography [J Chromatogr] 1991 May 03; Vol. 566 (1), pp. 127-34. |
| DOI: |
10.1016/0378-4347(91)80117-u |
| Abstrakt: |
A high-performance liquid chromatographic (HPLC) method with ultraviolet detection for the determination of a novel 4-aza-steroidal inhibitor of 5 alpha-reductase in human plasma has been developed. The assay is based on a single solid-phase extraction and an efficient HPLC separation on two analytical columns in series. The assay has been fully validated and used to support Phase II and III clinical pharmacokinetic studies. The lowest limit of quantification was found to be at 1 ng/ml and allowed pharmacokinetic evaluation of the drug at doses down to 5 mg. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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