| Autor: |
Jiang YZ; College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 625014, China., Li XW |
| Jazyk: |
angličtina |
| Zdroj: |
Yi chuan xue bao = Acta genetica Sinica [Yi Chuan Xue Bao] 2006 Feb; Vol. 33 (2), pp. 125-32. |
| DOI: |
10.1016/S0379-4172(06)60031-0 |
| Abstrakt: |
The intestinal fatty acid-binding protein (I-FABP) shows binding specificity for long-chain fatty acids and is proposed to be involved in the uptake of dietary fatty acids and their intracellular transport. In this study, the full-length cDNA of I-FABP was cloned from pig intestine by homology cloning approach combined with 3' and 5' RACE. Sequence analysis and bioinformatics study showed that this cDNA contained 614 nucleotides, with a 399 bp open reading frame (ORF) flanked by a 43 bp 5' UTR and a 172 bp 3' UTR. The encoded 132 amino acids of pig I-FABP with a molecular weight of approximately 15 kDa shared a high sequence identity of 68%-85% with those of other species. In addition, the phylogenetical analysis also indicated that the pig I-FABP was in the same branch with those of other species. The tissue-specific expression of pig I-FABP was measured by Northern hybridization and semi-quantitative RT-PCR. The results demonstrated that pig I-FABP mRNA was extensively present in various tissues, but I-FABP transcript of approximately 620 bp was more abundant in intestine than in other tissues. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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