| Autor: |
Shevchenko KV, Nagaev IIu, Alfeeva LIu, Andreeva LA, Kamenskiĭ AA, Levitskaia NG, Shevchenko VP, Grivennikov IA, Miasoedov NF |
| Jazyk: |
ruština |
| Zdroj: |
Bioorganicheskaia khimiia [Bioorg Khim] 2006 Jan-Feb; Vol. 32 (1), pp. 64-70. |
| DOI: |
10.1134/s1068162006010055 |
| Abstrakt: |
The radioactive peptide analogue Semax corresponding to the ACTH(4-10) sequence (Met-Glu-His-Phe-Pro-Gly-Pro) with a molar radioactivity of 56 Ci/mmol labeled with tritium at the C-terminal Pro was prepared. The labeled peptide was used for studying the kinetics of Semax penetration into rat brain and blood after its intranasal administration (50 microg/kg, 20 microl of solution) to nonbred white rats of body mass 200-250 g. It was demonstrated that 0.093% of the total introduced radioactivity per gram can be found in the rat brain 2 min after the administration, 80% of this radioactivity belonged to Semax, and the rest, to its metabolites. The peptide undergoes rapid enzymatic degradation, with the tripeptide Pro-Gly-Pro prevailing in biological samples relative to the total content of Semax and its metabolites. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
