| Autor: |
Li ZP; College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province, People's Republic of China. lzpbd@mail.hbu.edu.cn, Duan XR, Liu CH, Du BA |
| Jazyk: |
angličtina |
| Zdroj: |
Analytical biochemistry [Anal Biochem] 2006 Apr 01; Vol. 351 (1), pp. 18-25. Date of Electronic Publication: 2006 Feb 08. |
| DOI: |
10.1016/j.ab.2006.01.038 |
| Abstrakt: |
The interaction between cysteine and gold nanoparticles was studied. Through the covalent combination with the -SH group and the electrostatic binding with the -NH3+ group of cysteine, gold nanoparticles can self-assemble to form a network structure, which results in greatly enhanced resonance light scattering (RLS). The experimental results demonstrate that the RLS technique offers a sensitive tool for investigations of self-assembly of nanoparticles. On the other hand, the RLS method can be applied to selectively determine cysteine with high sensitivity and simple operation. The linear range of determination of cysteine is from 0.01 to 0.25 microg/mL with the detection limit of 2.0 ng/mL (16.5 nM, 3sigma). None of the amino acids found in proteins interferes with the determination. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Full Text from ScienceDirect