| Autor: |
Pfleger KD; 7TM Laboratory/Laboratory for Molecular Endocrinology, Western Australian Institute for Medical Research (WAIMR) and Centre for Medical Research, University of Western Australia, Nedlands, Perth, WA 6009, Australia. kpfleger@waimr.uwa.edu.au, Dromey JR, Dalrymple MB, Lim EM, Thomas WG, Eidne KA |
| Jazyk: |
angličtina |
| Zdroj: |
Cellular signalling [Cell Signal] 2006 Oct; Vol. 18 (10), pp. 1664-70. Date of Electronic Publication: 2006 Feb 21. |
| DOI: |
10.1016/j.cellsig.2006.01.004 |
| Abstrakt: |
Bioluminescence resonance energy transfer (BRET) is an increasingly popular technique for studying protein-protein interactions in live cells. It is particularly suitable for real-time monitoring of such interactions, however, the timescale over which assays can be carried out is currently relatively short (minutes) due to substrate instability. We present a new derivation of the BRET technology, termed 'extended BRET' (eBRET), which now enables protein-protein interactions to be monitored in real-time for many hours. This capability has significant benefits for investigating cellular function over extended timescales, as we have illustrated using the agonist-induced G-protein coupled receptor/beta-arrestin interaction. The potential for studying the modulation of such interactions by agonists, antagonists, inhibitors, dominant negative mutants and co-expressed accessory proteins is substantial. Furthermore, the advantages of eBRET have important implications for the development of high-throughput BRET screening systems, an ever-expanding area of interest for the pharmaceutical industry. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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