| Autor: |
Sorokin DY; Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/2, 117811 Moscow, Russia. D.Y.Sorokin@tnw.tudelft.nl, Zhilina TN, Lysenko AM, Tourova TP, Spiridonova EM |
| Jazyk: |
angličtina |
| Zdroj: |
Extremophiles : life under extreme conditions [Extremophiles] 2006 Jun; Vol. 10 (3), pp. 213-20. Date of Electronic Publication: 2006 Feb 21. |
| DOI: |
10.1007/s00792-005-0487-7 |
| Abstrakt: |
Four new isolates were obtained from denitrifying enrichments with various electron donors using sediment samples from hypersaline soda lakes. Based on 16S rRNA gene analysis and DNA-DNA hybridization results, they were all identified as members of the Gammaproteobacteria closely associated with the Alkalispirillum-Alkalilimnicola group. Two isolates were obtained from samples enriched with nitrate as electron acceptor and H2 or polysulfide as electron donors, and another two strains were obtained with N2O as the electron acceptor and sulfide or acetate as electron donors. All four new isolates, together with the type strains of the genera Alkalispirillum and Alkalilimnicola originally described as obligate aerobes, were capable of anaerobic growth with acetate using either nitrate or N2O as electron acceptors. Their denitrification pathway, however, was disrupted at the level of nitrite. RuBisCO form I gene was detected and sequenced in the new isolates and in Alkalilimnicola halodurans but not in Alkalispirillum mobile. These data, together with the evidence of Oremland et al. (Appl Environ Microbiol 68:4795-4802, 2002) on the potential of Alkalilimnicola sp. MLHE-1 for autotrophic growth with arsenite as electron donor and nitrate as electron acceptor, demonstrate much higher metabolic diversity of this specific group of haloalkaliphilic Gammaproteobacteria than was originally anticipated. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Full text from SpringerLink