| Autor: |
Birmingham A; Dharmacon Research, 2650 Crescent Drive, #100, Lafayette, Colorado 80026, USA., Anderson EM, Reynolds A, Ilsley-Tyree D, Leake D, Fedorov Y, Baskerville S, Maksimova E, Robinson K, Karpilow J, Marshall WS, Khvorova A |
| Jazyk: |
angličtina |
| Zdroj: |
Nature methods [Nat Methods] 2006 Mar; Vol. 3 (3), pp. 199-204. |
| DOI: |
10.1038/nmeth854 |
| Abstrakt: |
Off-target gene silencing can present a notable challenge in the interpretation of data from large-scale RNA interference (RNAi) screens. We performed a detailed analysis of off-targeted genes identified by expression profiling of human cells transfected with small interfering RNA (siRNA). Contrary to common assumption, analysis of the subsequent off-target gene database showed that overall identity makes little or no contribution to determining whether the expression of a particular gene will be affected by a given siRNA, except for near-perfect matches. Instead, off-targeting is associated with the presence of one or more perfect 3' untranslated region (UTR) matches with the hexamer or heptamer seed region (positions 2-7 or 2-8) of the antisense strand of the siRNA. These findings have strong implications for future siRNA design and the application of RNAi in high-throughput screening and therapeutic development. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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