Electrophoresis of DNA in human genetic diagnostics - state-of-the-art, alternatives and future prospects.

Autor: Gödde R; Department of Human Genetics, Ruhr-University, Bochum, Germany. rene.goedde@rub.de, Akkad DA, Arning L, Dekomien G, Herchenbach J, Kunstmann E, Meins M, Wieczorek S, Epplen JT, Hoffjan S
Jazyk: angličtina
Zdroj: Electrophoresis [Electrophoresis] 2006 Mar; Vol. 27 (5-6), pp. 939-46.
DOI: 10.1002/elps.200500675
Abstrakt: Electrophoretic separation of nucleic acids according to their molecular weights has dominated the methods' spectrum in molecular genetics for nearly half a century. We review the current methodological basis and evaluate its impact with special reference to new developments in the microarray technology. Although electrophoresis may be made redundant for many applications in DNA diagnostics within a few years, a number of electrophoretic vestiges will remain irreplaceable in the foreseeable future.
Databáze: MEDLINE