Electrochemically induced FTIR difference spectroscopy in the mid- to far infrared (200 microm) domain: a new setup for the analysis of metal-ligand interactions in redox proteins.

Autor: Berthomieu C; Laboratoire des Interactions Protéine Métal, DSV-DEVM, UMR 6191 CNRS-CEA-Univ. Aix-Marseille II, CEA-Cadarache, 13108 Saint Paul-lez-Durance Cedex, France. catherine.berthomieu@cea.fr, Marboutin L, Dupeyrat F, Bouyer P
Jazyk: angličtina
Zdroj: Biopolymers [Biopolymers] 2006 Jul; Vol. 82 (4), pp. 363-7.
DOI: 10.1002/bip.20469
Abstrakt: We report the setup of an electrochemical cell with chemical-vapor deposition diamond windows and the use of a Bruker 66 SX FTIR spectrometer equipped with DTGS and Si-bolometer detectors and KBr and mylar beam splitters, to record on the same sample, FTIR difference spectra corresponding to the structural changes associated with the change in redox state of active sites in proteins in the whole 1800-50 cm(-1) region. With cytochrome c we show that reliable reduced-minus-oxidized FTIR difference spectra are obtained, which correspond to single molecular vibrations. Redox-sensitive IR modes of the cytochrome c are detected until 140 cm(-1) with a good signal to noise. This new setup is promising to analyze the infrared spectral region where metal-ligand vibrations are expected to contribute and to extend the analysis of vibrational properties to metal sites or redox states not accessible to (resonance) Raman spectroscopy.
((c) 2006 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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