| Autor: |
Pinheiro TS; Núcleo de Investigações Químico-Farmacêuticas (Niqfar), Curso de Farmácia, CCS, University of Vale do Itajaí (Univali), Rua: Uruguai 458, Caixa Postal 360, Itajai, Santa Catarina (SC) 88302-202, Brazil., Johansson LA, Pizzolatti MG, Biavatti MW |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2006 May 03; Vol. 41 (2), pp. 431-6. Date of Electronic Publication: 2006 Jan 19. |
| DOI: |
10.1016/j.jpba.2005.12.010 |
| Abstrakt: |
The leaves of the pantropical genus Bauhinia (Fabaceae) are popularly known as cow's-paw or cow's hoof due to their unique characteristic bilobed aspect. The species Bauhinia forficata (Brazilian Orchid-tree) is widely used in folk medicine as an antidiabetic. This article deals with the quantitative analysis of kaempferitrin from B. forficata medicinal extract (aqueous and hydro alcoholic) using the LC method, and the comparison of kaempferitrin content in leaves collected from two different regions in the south Brazil. The total flavonoid content assessed by LC was also compared with the classical spectrophotometric determination. Kaempferitrin was found in different amounts, in samples from two geographical areas (Telêmaco Borba/PR and Itajaí/SC), for aqueous (368.68 and 77.91 microg/mL) and hydro alcoholic extracts (1952.59 and 211.61 microg/mL), respectively. The method was subjected to recovery assay, to determine its accuracy. A marked difference in total flavonoid concentration was observed in relation to kaempferitrin content: 2759.95 and 2188.20 microg/mL for the fluidextract and 863.35 and 856.77 microg/mL for the aqueous extract (Telêmaco Borba/PR and Itajaí/SC). The spectrophotometric assay overestimated the total flavonoid content (3620 microg/mL) in relation to the LC assay. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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