| Autor: |
Barbaroux JB; INSERM U255, IFR58, Centre de Recherches Biomédicales des Cordeliers, Université Pierre et Marie Curie (Paris 6) et René Descartes (Paris 5), Paris, France., Kwan WH, Allam JP, Novak N, Bieber T, Fridman WH, Groves R, Mueller CG |
| Jazyk: |
angličtina |
| Zdroj: |
The Journal of investigative dermatology [J Invest Dermatol] 2006 Jan; Vol. 126 (1), pp. 114-20. |
| DOI: |
10.1038/sj.jid.5700023 |
| Abstrakt: |
GM-CSF and transforming growth factor beta (TGFbeta ) are required for the generation of Langerhans cells (LC), members of the dendritic cell (DC) family. Tumor necrosis factor alpha (TNFalpha) and IL-4 can enhance LC differentiation from human monocytes or CD34(+) progenitors. Here, we show that M-CSF-cultured DC precursors derived from CD34(+) progenitors resemble dermal CD14(+) cells and readily convert to LC-like DC in GM-CSF/TGFbeta. The cells express Langerin, CD1a, and CCR6, migrate in response to CCR6 ligand CCL20, and contain Birbeck granules. TNFalpha and IL-4, added separately or together, have an inhibitory effect on LC differentiation. Cells differentiated in the presence of IL-4 and TNFalpha express low levels of CCR7. This suggests that M-CSF-conditioned DC precursors retain the capacity to efficiently undergo a differentiation program, giving rise to LC-like DC solely through the effect of GM-CSF and TGFbeta. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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