| Autor: |
Kim SB; Department of Biological Sciences, University of Ulsan, Nam-Gu, Ulsan 680-749, Republic of Korea., Kim JS, Lee JH, Yoon WJ, Lee DS, Ko MS, Kwon BS, Choi DH, Cho HR, Lee BJ, Chung DK, Lee HW, Park JW |
| Jazyk: |
angličtina |
| Zdroj: |
FEBS letters [FEBS Lett] 2006 Jan 09; Vol. 580 (1), pp. 311-8. Date of Electronic Publication: 2005 Dec 12. |
| DOI: |
10.1016/j.febslet.2005.12.012 |
| Abstrakt: |
This study demonstrates a requirement for NF-kappaB activation in cis-diamminedichloroplatinum (cisplatin)-induced apoptosis in human head and neck squamous cell carcinoma (HNSCC) cell lines. This conclusion was supported by the following observations: cisplatin induced IkappaBalpha degradation and NF-kappaB-dependent transcriptional activation prior to cell death; pyrrolidine dithiocarbamate (PDTC), a chemical inhibitor of NF-kappaB activation, prevented apoptosis; lactacystin, an inhibitor of IkappaBalpha degradation, also prevented apoptosis; and finally, the expression of a super-repressor mutant IkappaBalpha blocked apoptosis. The expression of tumor necrosis factor alpha (TNFalpha) was promoted by cisplatin treatment and was suppressed by PDTC treatment. In addition, a neutralizing antibody against TNFalpha protected cells from cisplatin-induced apoptosis. These findings suggest that NF-kappaB activation is required for cisplatin-induced apoptosis and TNFalpha may play an important role in NF-kappaB-mediated apoptosis in cisplatin-treated HNSCC cell lines. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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