JC virus DNA in cerebrospinal fluid samples from Brazilian AIDS patients with focal brain lesions without mass effect.

Autor: Fink MC; Laboratório de Virologia (LIM HC-FMUSP), Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, Rua Dr Enéas de Carvalho Aguiar, 470, São Paulo, Brazil., Penalva de Oliveira AC, Milagres FA, Vidal JE, Picerno-Pouza AF, Duarte Neto A, Pannuti CS
Jazyk: angličtina
Zdroj: The Journal of infection [J Infect] 2006 Jan; Vol. 52 (1), pp. 30-6.
DOI: 10.1016/j.jinf.2005.02.015
Abstrakt: Objective: To evaluate the presence of JC virus DNA in CSF samples from Brazilian AIDS patients with focal lesions of CNS white matter without mass effect compatible with progressive multifocal leukoencephalopathy (PML).
Methods: CSF samples from AIDS patients with neurological symptoms and a CT scan showing focal lesions of CNS white matter without mass effect suggestive of PML, and from AIDS and non-AIDS patients with non-PML neurological diseases were tested for JC virus DNA by PCR. The primers used to amplify the T antigen region of the JC virus resulted in a 173-bp fragment. The presence of the JC virus was confirmed by digestion of the PCR product using BamH1.
Results: The PCR for JCV DNA was negative in 119/120 non-PML CSF samples (specificity =99.2%). Of 56 CSF samples from AIDS patients with focal lesions of CNS white matter without mass effect, JCV DNA was positive in 48.2% (27/56). In 23/29 (79.3%) JCV DNA-negative cases, other causes for the encephalitic lesions were found. No JCV DNA-positive cases showed other diagnoses.
Conclusions: The prevalence of JCV DNA by PCR in CSF samples from Brazilian AIDS patients with focal brain lesions, without mass effect was 48.2%. In these patients, a negative JCV PCR is highly suggestive of other neurological conditions.
Databáze: MEDLINE
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