| Autor: |
Lee H; Division of Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada K1A OR6., To RJ, Latta RK, Biely P, Schneider H |
| Jazyk: |
angličtina |
| Zdroj: |
Applied and environmental microbiology [Appl Environ Microbiol] 1987 Dec; Vol. 53 (12), pp. 2831-4. |
| DOI: |
10.1128/aem.53.12.2831-2834.1987 |
| Abstrakt: |
The red yeast Rhodotorula mucilaginosa produced an esterase that accumulated in the culture supernatant on induction with triacetin. The enzyme was specific for substrates bearing an O-acetyl group, but was relatively nonspecific for the rest of the molecule, which could consist of a phenol, a monosaccharide, a polysaccharide, or an aliphatic alcohol. The esterase was more active against acetylxylan and glucose beta-d-pentaacetate than were a number of esterases from plant and animal sources, when activities on 4-nitrophenyl acetate were compared. The enzyme exhibited Michaelis-Menten kinetics and was active over a broad pH range (5.5 to 9.2), with an optimum between pH 8 and 10. In addition, the enzyme retained its activity for 2 h at 55 degrees C. The yeast that produced the enzyme did not produce xylanase and, hence, is of interest for the production of acetylxylan esterase that is free of xylanolytic activity. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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