| Autor: |
Ulmer DC; Lignocellulose Group, Department of Biotechnology, Swiss Federal Institute of Technology, ETH Honggerberg, CH-8093 Zurich, Switzerland., Leisola MS, Schmidt BH, Fiechter A |
| Jazyk: |
angličtina |
| Zdroj: |
Applied and environmental microbiology [Appl Environ Microbiol] 1983 Jun; Vol. 45 (6), pp. 1795-801. |
| DOI: |
10.1128/aem.45.6.1795-1801.1983 |
| Abstrakt: |
Phanerochaete chrysosporium degraded purified Kraft lignin, alkali-extracted and dioxane-extracted straw lignin, and lignosulfonates at a similar rate, producing small-molecular-weight ( approximately 1,000) soluble products which comprised 25 to 35% of the original lignins. At concentrations of 1 g of lignin liter, 90 to 100% of the acid-insoluble Kraft, alkali straw, and dioxane straw lignins were degraded by 1 g of fungal mycelium liter within an active ligninolytic period of 2 to 3 days. Cultures with biomass concentrations as low as 0.16 g liter could also completely degrade 1 g of lignin liter during an active period of 6 to 8 days. The absorbance at 280 nm of 2 g of lignosulfonate liter increased during the first 3 days of incubation and decreased to 35% of the original value during the next 7 days. The capacity of 1 g of cells to degrade alkali-extracted straw lignin under optimized conditions was estimated to be as high as 1.0 g day. This degradation occurred with a simultaneous glucose consumption rate of 1.0 g day. When glucose or cellular energy resources were depleted, lignin degradation ceased. The ability of P. chrysosporium to degrade the various lignins in a similar manner and at very low biomass concentrations indicates that the enzymes responsible for lignin degradation are nonspecific. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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