| Autor: |
Kuan KT; Department of Chemistry, University of Texas, Dallas, Richardson 75083-0688., Weber DS, Sottile L, Goux WJ |
| Jazyk: |
angličtina |
| Zdroj: |
Carbohydrate research [Carbohydr Res] 1992 Feb 17; Vol. 225 (1), pp. 123-36. |
| DOI: |
10.1016/0008-6215(92)80044-2 |
| Abstrakt: |
Methods are presented for the preparation of a variety of D-fructose phosphates, 13C-substituted at any single carbon site or at any two symmetrically disposed carbon sites, from either 13C-substituted pyruvate or L-alanine. It is demonstrated that millimole quantities of product can be obtained in good yield following a "one-pot" incubation of 13C-substituted precursors with commercially available enzymes and cofactors of the glycolytic pathway. Since it has previously been shown that a wide variety of aldehydes serve as acceptable substrates for the final rabbit muscle aldolase-catalyzed condensation step, the method can potentially be applied to prepare a wide variety of 13C-substituted sugars and sugar phosphates. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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