Sexual reproduction in subcultures of Absidia blakesleeana after years of preservation under mineral oil.

Autor: Santos MJ; Departamento de Bioquímica e Biologia Molecular, Instituto Oswaldo Cruz-IOC-FIOCRUZ, Rio de Janeiro, Brazil. mjsantos@ioc.fiocruz.br, Trufem SF, de Oliveira PC
Jazyk: angličtina
Zdroj: Revista iberoamericana de micologia [Rev Iberoam Micol] 2005 Sep; Vol. 22 (3), pp. 174-6.
DOI: 10.1016/s1130-1406(05)70036-x
Abstrakt: Two subcultures of Absidia blakesleeana IOC No. 2425 (-) mating type strain preserved in 1959 and 1981 on a thick layer of potato dextrose agar (PDA) medium under sterile mineral oil and maintained at room temperature in the Fungal Cultural Collection of Institute Oswaldo Cruz--IOC--were paired with the defined Absidia blakesleeana URM-UFP No. 2076 (+) mating type strain. Portions of the line where mycelia of the two strains met were observed under light microscopy. Zygospores belonging to Subgenus Mycocladus were observed. The results demonstrated conservation and re-establishment of the physiological and genetic metabolic processes of two subcultures preserved under mineral oil.
Databáze: MEDLINE