Understanding the response of dendritic cells to activation by streptococcal preparation OK-432.

Autor:	Kanzaki N; Department of Surgery 1, Fukushima Medical University, Fukushima 960-1295, Japan., Terashima M, Kashimura S, Hoshino M, Ohtani S, Matsuyama S, Hoshino Y, Kogure M, Oshibe I, Endo H, Saito T, Yaginuma H, Gotoh M, Ohto H
Jazyk:	angličtina
Zdroj:	Anticancer research [Anticancer Res] 2005 Nov-Dec; Vol. 25 (6B), pp. 4231-8.
Abstrakt:	Background: The streptococcal preparation OK-432 induces maturation of T cells and dendritic cells (DCs). However, the mechanisms by which OK-432 induces DC maturation are not well understood. Materials and Methods: The effects of OK-432 and TNF-alpha on peripheral blood mononuclear cells (PBMCs) were compared. Antibody-based approaches were used to detect proteins characteristic of antigen-presenting cells and cytokines. Cytotoxicity was evaluated by measuring the release of LDH after incubation of effector and target cells. The TLR-4 levels were measured by real-time quantitative PCR. Results: Changes in cell surface marker levels were detected in both treatment groups but OK-432 had a greater effect on induction of Th-1-type cytokines. Furthermore, TLR-4 mRNA was up-regulated in cells from two out of five patients in response to OK-432, but not in response to TNF-alpha. Conclusion: OK-432 has a more profound effect on human DCs than TNF-alpha and may act through multiple signaling pathways.
Databáze:	MEDLINE
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