Determination of 4-aminopyridine in serum by solid-phase extraction and high-performance liquid chromatography.

Autor: van der Horst A; Department of Pharmacy, Free University Hospital, Amsterdam, Netherlands., de Goede PN, van Diemen HA, Polman CH, Martens HJ
Jazyk: angličtina
Zdroj: Journal of chromatography [J Chromatogr] 1992 Feb 07; Vol. 574 (1), pp. 166-9.
DOI: 10.1016/0378-4347(92)80114-6
Abstrakt: An assay for the determination of 4-aminopyridine in serum has been developed using 3,4-diaminopyridine as internal standard and reversed-phase high-performance liquid chromatography with detection at 244 nm. A mobile phase of acetonitrile-methanol-ethanol-1% ammonium carbonate (75:10:10:5) provided excellent separation of both compounds. Samples were extracted on solid-phase columns. The linearity, precision, recovery and the limit of detection were all sufficient for the routine use of this assay in clinical studies of patients treated with 4-aminopyridine.
Databáze: MEDLINE