Autor: |
van der Horst A; Department of Pharmacy, Free University Hospital, Amsterdam, Netherlands., de Goede PN, van Diemen HA, Polman CH, Martens HJ |
Jazyk: |
angličtina |
Zdroj: |
Journal of chromatography [J Chromatogr] 1992 Feb 07; Vol. 574 (1), pp. 166-9. |
DOI: |
10.1016/0378-4347(92)80114-6 |
Abstrakt: |
An assay for the determination of 4-aminopyridine in serum has been developed using 3,4-diaminopyridine as internal standard and reversed-phase high-performance liquid chromatography with detection at 244 nm. A mobile phase of acetonitrile-methanol-ethanol-1% ammonium carbonate (75:10:10:5) provided excellent separation of both compounds. Samples were extracted on solid-phase columns. The linearity, precision, recovery and the limit of detection were all sufficient for the routine use of this assay in clinical studies of patients treated with 4-aminopyridine. |
Databáze: |
MEDLINE |
Externí odkaz: |
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