| Autor: |
Zhu S; State Key Laboratory for Biocontrol and Center for Parasitic Organisms, School of Life Sciences, Sun Yat-Sen, Zhongshan University, Guangzhou 510275, PR China., Lai DH, Li SQ, Lun ZR |
| Jazyk: |
angličtina |
| Zdroj: |
Cell biology international [Cell Biol Int] 2006 Feb; Vol. 30 (2), pp. 149-53. Date of Electronic Publication: 2005 Nov 02. |
| DOI: |
10.1016/j.cellbi.2005.09.004 |
| Abstrakt: |
The influence of insulin and 2-deoxy-glucose (D-glucose) on the intracellular protozoan Toxoplasma gondii replication in 3T3-L1 cells was investigated. Insulin and D-glucose had a dose-responsive mitogenic effect on intracellular T. gondii replication and development in 3T3-L1 cells. Insulin concentrations between 10(-2) and 10(-1) microg/ml combination of 4.5 g/l D-glucose in DMEM medium gave maximum stimulus to T. gondii replication. The number of tachyzoites increased rapidly, with the growth peaking typically on day 3 or 4 of culture, and then declining quickly. However, insulin, in the absence of d-glucose, had comparably less effect on T. gondii growth than two of their combination. d-glucose concentrations significantly affected the tachyzoite replication and appear to be indispensable for maintaining the host 3T3-L1 cells. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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